PA5-77524
antibody from Invitrogen Antibodies
Targeting: NECTIN1
CD111, CLPED1, ED4, HIgR, HVEC, OFC7, PRR, PRR1, PVRL1, PVRR1, SK-12
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-77524 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nectin 1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates with Nectin 1 (extracellular) polyclonal antibody (Product # PA5-77524) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates with Nectin 1 (extracellular) polyclonal antibody (Product # PA5-77524) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates with Nectin 1 (extracellular) polyclonal antibody (Product # PA5-77524) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Nectin 1 (extracellular) Polyclonal Antibody (Product # PA5-77524) and a 55 kDa band corresponding to Nectin-1 was observed only in Mouse and Rat Brain which are reported to be positive and not in any other tissues. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Skeletal Muscle (Lane 3) and Mouse Heart (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:200 dil) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005). An uncharacterized band of ~30 kDa was also observed in Mouse Heart.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Nectin 1 (extracellular) in rat PC12 pheochromocytoma cells. A) Samples were probed with Nectin 1 (extracellular) polyclonal antibody (Product # PA5-77524) using a dilution of 1:100, and incubated with goat-anti-rabbit-AlexaFluor-594. B) Live view image. C) Merged image of Panels A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nectin 1 (extracellular) in immersion-fixed, free floating, frozen rat brain. A) Samples were probed with Nectin 1 (extracellular) polyclonal antibody (Product # PA5-77524) using a dilution of 1:100, and incubated with DAPI (red). B) Stained nuclei (blue). C) Merged image of Panels A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of human THP-1 monocytic leukemia cells using polyclonal Nectin 1 (extracellular) antibody (Product # PA5-77524) with a dilution of 1:15 followed by a goat anti-rabbit-AlexaFluor-488 secondary antibody.