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antibody from Invitrogen Antibodies
Targeting: NECTIN1
CD111, CLPED1, ED4, HIgR, HVEC, OFC7, PRR, PRR1, PVRL1, PVRR1, SK-12
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- PA5-85668 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nectin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (90%), Rat (89%), Dog (95%), Pig (96%). Positive Control: mouse brain, rat brain. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.33 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nectin 1 in whole cell extract using Nectin 1 polyclonal antibody (Product # PA5-85668) using 30 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nectin 1 in various tissue extracts using Nectin 1 polyclonal antibody (Product # PA5-85668) using 50 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Nectin 1 Polyclonal Antibody (Product # PA5-85668). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PVRL1 antibody Nectin 1 Polyclonal Antibody (Product # PA5-85668) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Nectin 1 Polyclonal Antibody (Product # PA5-85668). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nectin 1 Polyclonal Antibody (Product # PA5-85668) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Nectin 1 Polyclonal Antibody detects Nectin 1 protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nectin 1 protein stained by Nectin 1 Polyclonal Antibody (Product # PA5-85668) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta-3 Tubulin Polyclonal Antibody [GT1338]diluted at 1:500. Blue: Fluoroshield with DAPI .
