Explore
Validate
LearnRQ5899
antibody from NSJ Bioreagents
Targeting: NECTIN1
CD111, CLPED1, ED4, HIgR, HVEC, OFC7, PRR, PRR1, PVRL1, PVRR1, SK-12
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- RQ5899 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Nectin 1 Antibody / PVRL1
- Antibody type
- Polyclonal
- Description
- This highly specific Nectin 1 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Nectin 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HEK293, 2) CCRF-CEM, 3) SW620, 4) U-87 MG, 5) SGC-7901, 6) Jurkat, 7) rat PC-12, 8) mouse spleen, 9) mouse NIH 3T3 and 10) mouse RAW264.7 lysate with Nectin 1 antibody. Predicted molecular weight ~110 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human rectal cancer with Nectin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human rectal cancer with Nectin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat brain with Nectin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat brain with Nectin 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human PC-3 cells with Nectin 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nectin 1 antibody.
