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LearnANR-051-200UL
antibody from Invitrogen Antibodies
Targeting: NECTIN1
CD111, CLPED1, ED4, HIgR, HVEC, OFC7, PRR, PRR1, PVRL1, PVRR1, SK-12
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ANR-051-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nectin-1/PVRL1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051), (1:200).3,4. Anti-Nectin-1/PVRL1 (extracellular) Antibody , preincubated with Nectin-1/PVRL1 (extracellular) Blocking Peptide (#BLP-NR051).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: - 1,2. Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051), (1:200).3,4. Anti-Nectin-1/PVRL1 (extracellular) Antibody , preincubated with Nectin-1/PVRL1 (extracellular) Blocking Peptide (#BLP-NR051).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat T-cell leukemia (lanes 1 and 3) and human MEG-01 chronic myelogenous leukemia (lanes 2 and 4) cell lysates: - 1,2. Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051), (1:200).3,4. Anti-Nectin-1/PVRL1 (extracellular) Antibody , preincubated with Nectin-1/PVRL1 (extracellular) Blocking Peptide (#BLP-NR051).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Nectin-1 in rat PC12 cells - Cell surface detection of Nectin-1 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051), (1:100), followed by goat Anti-rabbit-AlexaFluor-594secondary Antibody (red). B. Live image of the cells. C. Merge of the two images.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of Nectin-1 in rat cerebellum - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051), (1:100). A. Nectin-1staining (red) is apparent in Purkinje neurons and their dendritic tree (arrow). B. Cell nuclei in the same section are visualized with DAPI (blue). C. Merge of the two images.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of Nectin-1 by indirect flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Nectin-1/PVRL1 (extracellular) Antibody (#ANR-051), (2.5μg) + goat- Anti-rabbit-FITC.
