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LearnPA5-77823
antibody from Invitrogen Antibodies
Targeting: ING1
p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- PA5-77823 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ING1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ING1 in mouse brain cell lysates. The sample was incubated with ING1 polyclonal antibody (Product # PA5-77823) using a dilution of 1:1000. Samples were arranged as follows: Left) cell expressed flag-tag, Right) Untagged.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ING1 in human heat shocked HeLa cells. Sample was fixed using 2% formaldehyde (20 min at RT), incubated with ING1 polyclonal antibody (Product # PA5-77823) using a dilution of 1:50 (12 hours at 4°C), and followed by APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. Images are as follows: (A) DAPI (blue) nuclear stain. (B) Anti-p33 ING1 Antibody. (C) Composite. Heat Shocked at 42°C for 30 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ING1 in human heat shocked HeLa cells. Sample was fixed with 2% formaldehyde (20 min at RT), incubated with ING1 polyclonal antibody (Product # PA5-77823) using a dilution of 1:50 (12 hours at 4°C), and followed by FITC Goat Anti-Rabbit (green) and DAPI secondary antibody at a dilution of 1:200 and 1:40,000 (2 hours at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, (B) Anti-p33 ING1 Antibody, (C) Merged image. Magnification: 100x.
