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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- RQ5666 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Hepatic Lipase Antibody / LIPC
- Antibody type
- Polyclonal
- Description
- This highly specific Hepatic Lipase antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Direct ELISA applications with human and rat samples.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the Hepatic Lipase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of rat liver lysate with Hepatic Lipase antibody. Predicted molecular weight ~56 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human HepG2 cells with Hepatic Lipase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver cancer with Hepatic Lipase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat liver with Hepatic Lipase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
