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- Antigen structure
- References [12]
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- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- 11931-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11931-1-AP, RRID:AB_2087897
- Product name
- CSPP1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated CSPP1 antibody (Cat. #11931-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Fixation methods and immunolabeling for cilia proteins in ciliary and extraciliary locations.
The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.
CK1-mediated phosphorylation of FAM110A promotes its interaction with mitotic spindle and controls chromosomal alignment.
Rpgrip1l controls ciliary gating by ensuring the proper amount of Cep290 at the vertebrate transition zone.
A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.
The E3 ubiquitin ligase UBR5 regulates centriolar satellite stability and primary cilia.
Fixation methods can differentially affect ciliary protein immunolabeling.
CSPP-L Associates with the Desmosome of Polarized Epithelial Cells and Is Required for Normal Spheroid Formation.
Nuclear CSPP1 expression defined subtypes of basal-like breast cancer.
Mutations in CSPP1 lead to classical Joubert syndrome.
Mutations in CSPP1 cause primary cilia abnormalities and Joubert syndrome with or without Jeune asphyxiating thoracic dystrophy.
CSPP is a ciliary protein interacting with Nephrocystin 8 and required for cilia formation.
Hua K, Ferland RJ
Methods in cell biology 2023;176:43-57
Methods in cell biology 2023;176:43-57
The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.
Batman U, Deretic J, Firat-Karalar EN
PLoS biology 2022 Jul;20(7):e3001708
PLoS biology 2022 Jul;20(7):e3001708
CK1-mediated phosphorylation of FAM110A promotes its interaction with mitotic spindle and controls chromosomal alignment.
Aquino Perez C, Burocziova M, Jenikova G, Macurek L
EMBO reports 2021 Jul 5;22(7):e51847
EMBO reports 2021 Jul 5;22(7):e51847
Rpgrip1l controls ciliary gating by ensuring the proper amount of Cep290 at the vertebrate transition zone.
Wiegering A, Dildrop R, Vesque C, Khanna H, Schneider-Maunoury S, Gerhardt C
Molecular biology of the cell 2021 Apr 15;32(8):675-689
Molecular biology of the cell 2021 Apr 15;32(8):675-689
A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.
Frikstad KM, Molinari E, Thoresen M, Ramsbottom SA, Hughes F, Letteboer SJF, Gilani S, Schink KO, Stokke T, Geimer S, Pedersen LB, Giles RH, Akhmanova A, Roepman R, Sayer JA, Patzke S
Cell reports 2019 Aug 13;28(7):1907-1922.e6
Cell reports 2019 Aug 13;28(7):1907-1922.e6
The E3 ubiquitin ligase UBR5 regulates centriolar satellite stability and primary cilia.
Shearer RF, Frikstad KM, McKenna J, McCloy RA, Deng N, Burgess A, Stokke T, Patzke S, Saunders DN
Molecular biology of the cell 2018 Jul 1;29(13):1542-1554
Molecular biology of the cell 2018 Jul 1;29(13):1542-1554
Fixation methods can differentially affect ciliary protein immunolabeling.
Hua K, Ferland RJ
Cilia 2017;6:5
Cilia 2017;6:5
CSPP-L Associates with the Desmosome of Polarized Epithelial Cells and Is Required for Normal Spheroid Formation.
Sternemalm J, Geimer S, Frikstad KA, Schink KO, Stokke T, Patzke S
PloS one 2015;10(8):e0134789
PloS one 2015;10(8):e0134789
Nuclear CSPP1 expression defined subtypes of basal-like breast cancer.
Sternemalm J, Russnes HG, Zhao X, Risberg B, Nord S, Caldas C, Børresen-Dale AL, Stokke T, Patzke S
British journal of cancer 2014 Jul 15;111(2):326-38
British journal of cancer 2014 Jul 15;111(2):326-38
Mutations in CSPP1 lead to classical Joubert syndrome.
Akizu N, Silhavy JL, Rosti RO, Scott E, Fenstermaker AG, Schroth J, Zaki MS, Sanchez H, Gupta N, Kabra M, Kara M, Ben-Omran T, Rosti B, Guemez-Gamboa A, Spencer E, Pan R, Cai N, Abdellateef M, Gabriel S, Halbritter J, Hildebrandt F, van Bokhoven H, Gunel M, Gleeson JG
American journal of human genetics 2014 Jan 2;94(1):80-6
American journal of human genetics 2014 Jan 2;94(1):80-6
Mutations in CSPP1 cause primary cilia abnormalities and Joubert syndrome with or without Jeune asphyxiating thoracic dystrophy.
Tuz K, Bachmann-Gagescu R, O'Day DR, Hua K, Isabella CR, Phelps IG, Stolarski AE, O'Roak BJ, Dempsey JC, Lourenco C, Alswaid A, Bönnemann CG, Medne L, Nampoothiri S, Stark Z, Leventer RJ, Topçu M, Cansu A, Jagadeesh S, Done S, Ishak GE, Glass IA, Shendure J, Neuhauss SC, Haldeman-Englert CR, Doherty D, Ferland RJ
American journal of human genetics 2014 Jan 2;94(1):62-72
American journal of human genetics 2014 Jan 2;94(1):62-72
CSPP is a ciliary protein interacting with Nephrocystin 8 and required for cilia formation.
Patzke S, Redick S, Warsame A, Murga-Zamalloa CA, Khanna H, Doxsey S, Stokke T
Molecular biology of the cell 2010 Aug 1;21(15):2555-67
Molecular biology of the cell 2010 Aug 1;21(15):2555-67
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 11931-1-AP(CSPP1 antibody) at dilution of 1:200
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using CSPP1 antibody 11931-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
