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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- PA5-49336 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HBG2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HBG2 in lysates from human brain, mouse brain and eyeball tissue lysate (from left to right, 35µg per lane). Samples were probed using a HBG2 Antibody (C-term) (Product # PA5-49336) at a 1:1000 dilution, followed by a goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HBG2 in paraffin-embedded Human liver tissue. Samples were probed using a HBG2 Antibody (C-term) (Product # PA5-49336) at a 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at a 1:400 dilution was used as the secondary antibody, followed by DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HBG2 in paraffin-embedded Mouse liver section. Samples were incubated using HBG2 Antibody (C-term) (Product # PA5-49336) at a 1:100 dilution, followed by a peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HBG2 in U-87 MG cells. Cells were incubated with a HBG2 Antibody (C-term) (green) (Product # PA5-49336) at a 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody. Isotype control of rabbit IgG (blue).
