Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB6130-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse GM-CSF R alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse GM-CSF R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or rhGM-CSF R beta is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q00941
- Isotype
- IgG
- Antibody clone number
- 698423
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Essential Role of mTORC1 in Self-Renewal of Murine Alveolar Macrophages.
Frontline Science: Coincidental null mutation of Csf2rα in a colony of PI3Kγ-/- mice causes alveolar macrophage deficiency and fatal respiratory viral infection.
Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis.
Deng W, Yang J, Lin X, Shin J, Gao J, Zhong XP
Journal of immunology (Baltimore, Md. : 1950) 2017 Jan 1;198(1):492-504
Journal of immunology (Baltimore, Md. : 1950) 2017 Jan 1;198(1):492-504
Frontline Science: Coincidental null mutation of Csf2rα in a colony of PI3Kγ-/- mice causes alveolar macrophage deficiency and fatal respiratory viral infection.
Schneider C, Nobs SP, Heer AK, Hirsch E, Penninger J, Siggs OM, Kopf M
Journal of leukocyte biology 2017 Feb;101(2):367-376
Journal of leukocyte biology 2017 Feb;101(2):367-376
Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis.
Weber GF, Chousterman BG, Hilgendorf I, Robbins CS, Theurl I, Gerhardt LM, Iwamoto Y, Quach TD, Ali M, Chen JW, Rothstein TL, Nahrendorf M, Weissleder R, Swirski FK
The Journal of experimental medicine 2014 Jun 2;211(6):1243-56
The Journal of experimental medicine 2014 Jun 2;211(6):1243-56
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- GM-CSF R alpha in J774A.1 Mouse Cell Line. GM-CSF R alpha was detected in immersion fixed J774A.1 mouse reticulum cell sarcoma macrophage cell line using Rat Anti-Mouse GM-CSF R alpha Mono-clonal Antibody (Catalog # MAB6130) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of GM-CSF R alpha in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse GM-CSF R alpha Monoclonal Antibody (Catalog # MAB6130, filled histo-gram) or isotype control antibody (Catalog # MAB006, open histo-gram), followed by Allophy-cocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by GM-CSF and Neutralization by Mouse GM-CSF R alpha Antibody. Recombinant Mouse GM-CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM-CSF (0.1 ng/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse GM-CSF R alpha Monoclonal Anti-body (Catalog # MAB6130). The ND50 is typically 0.02-0.12 µg/mL.