Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-1169-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD116 Monoclonal Antibody (4H1), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 4H1 monoclonal antibody reacts with the human CD116 molecule, the alpha subunit of GM-CSF receptor. The alpha subunit associates with the common beta chain (CD131) to form the high affinity receptor for GM-CSF. The GM-CSFR alpha chain is expressed by granulocytes, monocytes, endothelial cells, fibroblasts and some tumor cells. Applications Reported: 4H1 has been reported for use in flow cytometric analysis. Applications Tested: The 4H1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4H1
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Highly variable cancer subpopulations that exhibit enhanced transcriptome variability and metastatic fitness.
IL-15 trans-presentation promotes human NK cell development and differentiation in vivo.
Simultaneous antagonism of interleukin-5, granulocyte-macrophage colony-stimulating factor, and interleukin-3 stimulation of human eosinophils by targetting the common cytokine binding site of their receptors.
The human granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor exists as a preformed receptor complex that can be activated by GM-CSF, interleukin-3, or interleukin-5.
Interleukin-5, interleukin-3, and granulocyte-macrophage colony-stimulating factor cross-compete for binding to cell surface receptors on human eosinophils.
Nguyen A, Yoshida M, Goodarzi H, Tavazoie SF
Nature communications 2016 May 3;7:11246
Nature communications 2016 May 3;7:11246
IL-15 trans-presentation promotes human NK cell development and differentiation in vivo.
Huntington ND, Legrand N, Alves NL, Jaron B, Weijer K, Plet A, Corcuff E, Mortier E, Jacques Y, Spits H, Di Santo JP
The Journal of experimental medicine 2009 Jan 16;206(1):25-34
The Journal of experimental medicine 2009 Jan 16;206(1):25-34
Simultaneous antagonism of interleukin-5, granulocyte-macrophage colony-stimulating factor, and interleukin-3 stimulation of human eosinophils by targetting the common cytokine binding site of their receptors.
Sun Q, Jones K, McClure B, Cambareri B, Zacharakis B, Iversen PO, Stomski F, Woodcock JM, Bagley CJ, D'Andrea R, Lopez AF
Blood 1999 Sep 15;94(6):1943-51
Blood 1999 Sep 15;94(6):1943-51
The human granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor exists as a preformed receptor complex that can be activated by GM-CSF, interleukin-3, or interleukin-5.
Woodcock JM, McClure BJ, Stomski FC, Elliott MJ, Bagley CJ, Lopez AF
Blood 1997 Oct 15;90(8):3005-17
Blood 1997 Oct 15;90(8):3005-17
Interleukin-5, interleukin-3, and granulocyte-macrophage colony-stimulating factor cross-compete for binding to cell surface receptors on human eosinophils.
Lopez AF, Vadas MA, Woodcock JM, Milton SE, Lewis A, Elliott MJ, Gillis D, Ireland R, Olwell E, Park LS
The Journal of biological chemistry 1991 Dec 25;266(36):24741-7
The Journal of biological chemistry 1991 Dec 25;266(36):24741-7
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Surface staining of normal human peripheral blood cells with Anti-Human CD116 FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.