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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-48256 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GFER Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Augmenter of liver regeneration protects the kidney from ischaemia-reperfusion injury in ferroptosis.
Huang LL, Liao XH, Sun H, Jiang X, Liu Q, Zhang L
Journal of cellular and molecular medicine 2019 Jun;23(6):4153-4164
Journal of cellular and molecular medicine 2019 Jun;23(6):4153-4164
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GFER in mouse NIH-3T3 cell line lysates (35 µg/lane). Lysates were probed with a GFER Antibody (C-term) (Product # PA5-48256).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of GFER in HepG2 cells (right histogram) compared to a negative control cell (left histogram). Cells were probed with a GFER Antibody (C-term) (Product # PA5-48256). Following incubation with the primary antibody, cells were incubated with FITC-conjugated goat-anti-rabbit secondary antibodies.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Ferroptosis was more apparent at H6R12 h, and augmenter of liver regeneration ( ALR ) was up-regulated after I/R injury. A, Representative Western blot demonstrating the expression of acyl-CoA synthetase long-chain family member 4 ( ACSL 4), transferrin receptor ( TFR ), SLC 7A11, glutathione peroxidase ( GPX ) 4 and ALR . B, The ratio of ACSL 4, TFR , SLC 7A11, GPX 4 and ALR was quantified by densitometry based on immunoblot images and depicted as the radio of the indicated proteins normalized to beta-actin. * P < 0.05 and ** P < 0.01 compared to the normal group
