PA5-48256

antibody from Invitrogen Antibodies

Targeting: GFER ALR, ERV1, HERV1, HPO1, HPO2, HSS

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-48256

Provider

Invitrogen Antibodies

Product name

GFER Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Augmenter of liver regeneration protects the kidney from ischaemia-reperfusion injury in ferroptosis.

Huang LL, Liao XH, Sun H, Jiang X, Liu Q, Zhang L
Journal of cellular and molecular medicine 2019 Jun;23(6):4153-4164

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of GFER in HepG2 cells (right histogram) compared to a negative control cell (left histogram). Cells were probed with a GFER Antibody (C-term) (Product # PA5-48256). Following incubation with the primary antibody, cells were incubated with FITC-conjugated goat-anti-rabbit secondary antibodies.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Ferroptosis was more apparent at H6R12 h, and augmenter of liver regeneration ( ALR ) was up-regulated after I/R injury. A, Representative Western blot demonstrating the expression of acyl-CoA synthetase long-chain family member 4 ( ACSL 4), transferrin receptor ( TFR ), SLC 7A11, glutathione peroxidase ( GPX ) 4 and ALR . B, The ratio of ACSL 4, TFR , SLC 7A11, GPX 4 and ALR was quantified by densitometry based on immunoblot images and depicted as the radio of the indicated proteins normalized to beta-actin. * P < 0.05 and ** P < 0.01 compared to the normal group