Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-85562 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CITED2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CITED2 in A) 293T whole cell lysate, B) HeLa whole cell lysate, C) HepG2 whole cell lysate using CITED2 polyclonal antibody (Product # PA5-85562) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CITED2 Polyclonal Antibody detects CITED2 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg HeLa whole cell lysate/extract. C. 30 µg HepG2 whole cell lysate/extract.12% SDS-PAGE. CITED2 Polyclonal Antibody (Product # PA5-85562) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CITED2 Polyclonal Antibody detects CITED2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CITED2 protein stained by CITED2 Polyclonal Antibody (Product # PA5-85562) diluted at 1:1,000. Blue: Hoechst 33342 staining.