MA1-10359

antibody from Invitrogen Antibodies

Targeting: HLA-G

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-10359

Provider

Invitrogen Antibodies

Product name

HLA-G Monoclonal Antibody (01G)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody recognizes membrane-bound form of HLA-G (full-length HLA-G1), but not soluble forms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. It will not cross-react with mouse or rat.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

01G

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C, do not freeze

References

Submitted references

Mesenchymal stromal cell delivery as a potential therapeutic strategy against COVID-19: Promising evidence from in vitro results.

Mallis P, Chatzistamatiou T, Dimou Z, Sarri EF, Georgiou E, Salagianni M, Triantafyllia V, Andreakos E, Stavropoulos-Giokas C, Michalopoulos E
World journal of biological chemistry 2022 Mar 27;13(2):47-65

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Evaluation of HLA-G Expression in Multipotent Mesenchymal Stromal Cells Derived from Vitrified Wharton's Jelly Tissue.

Mallis P, Boulari D, Michalopoulos E, Dinou A, Spyropoulou-Vlachou M, Stavropoulos-Giokas C
Bioengineering (Basel, Switzerland) 2018 Nov 1;5(4)

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry surface staining pattern of HLA-G transfected LCL cells using anti-human HLA-G (01G) purified Monoclonal antibody (Product # MA1-10359) (concentration in sample 16 µg/mL) GAM APC.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Separation of HLA-G transfected LCL cells (red-filled) from K562 cells (black-dashed) in flow cytometry analysis (surface staining) using anti-human HLA-G (01G) purified Monoclonal antibody (Product # MA1-10359) (concentration in sample 16 µg/mL) GAM APC.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Comprehensive characterization of characteristics of stimulated Wharton's Jelly and bone marrow mesenchymal stromal cells. A: Experimental workflow; B: Morphological analysis of characteristics of unstimulated and stimulated Wharton's Jelly (WJ) and bone marrow-mesenchymal stromal cells (BM-MSCs). Original magnification 20 x, scale bars = 50 mum; C: Determination of cell proliferation and viability. Statistically significant differences were observed in cell proliferation between stimulated and unstimulated WJ-MSCs ( P < 0.05) and stimulated and unstimulated BM-MSCs ( P < 0.05). No statistically significant difference was observed in cell viability either in an unstimulated or stimulated state ( P = 0.873); D: Immunophenotypic analysis of stimulated and unstimulated WJ and BM-MSCs. Over 95% of WJ and BM-MSCs in both states expressed CD73, CD90, CD105, CD29, and CD340, and less than 2% expressed CD34 and CD45; E: Determination of HLA-G isoforms (HLA-G1, G5, and G7) in unstimulated and stimulated MSCs from both sources; F: Indirect immunofluorescence against HLA-G1 in combination with DAPI stain was performed on unstimulated and stimulated WJ and BM-MSCs. Original magnification 63 x, scale bars = 10 mum. BM: Bone marrow; MSCs: Mesenchymal stromal cells; WJ: Wharton's Jelly.