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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- OAAB05537 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- HLA-G antibody - center region
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of HLA-G (arrow) using rabbit polyclonal HLA-G Antibody (Center) either nontransfected (Lane 1) or lysates transiently transfected transfected (Lane 2) with the HLA-G gene.
- Sample type
- lysatestransiently transfected with the HLA-G gene.
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of HLA-G Antibody (Center) in NCI-H460 cell lysates (35ug/lane). HLA-G (arrow) was detected using the purified Pab.
- Sample type
- NCI-H460 cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HLA-G Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Sample type
- human lung carcinoma
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- HLA-G Antibody (Center) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- NCI-H460 cells
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
