GTX80253

antibody from GeneTex

Targeting: HLA-G

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Functional assay (Data presented on provider website)

Antibody Data

Product number

GTX80253

Provider

GeneTex

Proper citation

GeneTex Cat#GTX80253, RRID:AB_11177049

Product name

HLA-G antibody [87G] (Biotin)

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Analysis of cytolytical activity of human polyclonal NK cells on target melanoma cells. Blocking of HLA-G1 on transfectants with anti-human HLA-G (87G) restored specific lysis. Target cells: M8 cell line transfected with empty vector (column 1) and with HLA-G1 cDNA (columns 2-6).Blocking antibodies: Column 1-2: noneColumn 3: Isotype mouse IgG2a controlColumn 4: anti-human HLA-G (87G) purifiedColumn 5: anti-human HLA-G (87G) F(ab')2 fragmentColumn 6: anti-human HLA-G (MEM-G/9)