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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F51441 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- HLA-G Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 62-89 from the human protein was used as the immunogen for this HLA-G antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the HLA-G antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of HLA-G antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the HLA-G gene.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of HLA-G antibody and NCI-H460 lysate. Predicted molecular weight ~40 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from human placenta tissue lysate using HLA-G antibody diluted at 1:1000.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human lung carcinoma stained with HLA-G antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- HLA-G antibody flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
