Antibody data
- Antibody Data
 - Antigen structure
 - References [3]
 - Comments [0]
 - Validations
 - Immunocytochemistry [1]
 - Immunoprecipitation [1]
 - Flow cytometry [1]
 
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Validation data
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- Product number
 - MAB3855 - Provider product page

 - Provider
 - Abnova Corporation
 - Proper citation
 - Abnova Corporation Cat#MAB3855, RRID:AB_10562334
 - Product name
 - HLA-G monoclonal antibody, clone MEM-G/9
 - Antibody type
 - Monoclonal
 - Description
 - Mouse monoclonal antibody raised against recombinant HLA-G.
 - Isotype
 - IgG
 - Antibody clone number
 - MEM-G/9
 - Storage
 - Store at 4°C. Do not freeze.Aliquot to avoid repeated freezing and thawing.
 
Submitted references		Regulatory role of tryptophan degradation pathway in HLA-G expression by human monocyte-derived dendritic cells.
				
The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules.
				
Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
				
		
	
			López AS, Alegre E, LeMaoult J, Carosella E, González A
Molecular immunology 2006 Jul;43(14):2151-60
		Molecular immunology 2006 Jul;43(14):2151-60
The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules.
			Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsí V, Goldman-Wohl D, Yagel S, Mandelboim O
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):4866-74
		Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):4866-74
Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
			Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N
Human immunology 2003 Mar;64(3):315-26
		Human immunology 2003 Mar;64(3):315-26
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					Supportive validation
					
									
				
				- Submitted by
 - Abnova Corporation (provider)
 - Main image
 
- Experimental details
 - Immunofluorescence staining of HLA-G1 transfectants (LCL-HLA-G1) using HLA-G monoclonal antibody, clone MEM-G/9 (Cat # MAB3855) Alexa Fluor® 488 Fab-fragment.
 - Validation comment
 - Immunofluorescence
 
							
					Supportive validation
					
									
				
				- Submitted by
 - Abnova Corporation (provider)
 - Main image
 
- Experimental details
 - Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by HLA-G monoclonal antibody, clone MEM-G/9 (Cat # MAB3855) and protein G. HLA-G was detected by HLA-G monoclonal antibody, clone 4H84 (Cat # MAB3858) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).
 - Validation comment
 - Immunoprecipitation
 
							
					Supportive validation
					
									
				
		- Submitted by
 - Abnova Corporation (provider)
 - Main image
 
- Experimental details
 - Double surface staining of HLA-G1 transfectants (viable cells gate) using HLA-G monoclonal antibody, clone MEM-G/9 (Cat # MAB3855) - APC and HLA-G monoclonal antibody, clone MEM-G/11 (Cat # MAB3856) - FITC.
 - Validation comment
 - Flow Cytometry