Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB3855 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB3855, RRID:AB_10562334
- Product name
- HLA-G monoclonal antibody, clone MEM-G/9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant HLA-G.
- Isotype
- IgG
- Antibody clone number
- MEM-G/9
- Storage
- Store at 4°C. Do not freeze.Aliquot to avoid repeated freezing and thawing.
Submitted references Regulatory role of tryptophan degradation pathway in HLA-G expression by human monocyte-derived dendritic cells.
The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules.
Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
López AS, Alegre E, LeMaoult J, Carosella E, González A
Molecular immunology 2006 Jul;43(14):2151-60
Molecular immunology 2006 Jul;43(14):2151-60
The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules.
Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsí V, Goldman-Wohl D, Yagel S, Mandelboim O
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):4866-74
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):4866-74
Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N
Human immunology 2003 Mar;64(3):315-26
Human immunology 2003 Mar;64(3):315-26
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence staining of HLA-G1 transfectants (LCL-HLA-G1) using HLA-G monoclonal antibody, clone MEM-G/9 (Cat # MAB3855) Alexa Fluor® 488 Fab-fragment.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by HLA-G monoclonal antibody, clone MEM-G/9 (Cat # MAB3855) and protein G. HLA-G was detected by HLA-G monoclonal antibody, clone 4H84 (Cat # MAB3858) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).
- Validation comment
- Immunoprecipitation
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Double surface staining of HLA-G1 transfectants (viable cells gate) using HLA-G monoclonal antibody, clone MEM-G/9 (Cat # MAB3855) - APC and HLA-G monoclonal antibody, clone MEM-G/11 (Cat # MAB3856) - FITC.
- Validation comment
- Flow Cytometry