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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunoprecipitation [1]
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- Product number
- MAB3858 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB3858, RRID:AB_10553670
- Product name
- HLA-G monoclonal antibody, clone 4H84
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against HLA-G .
- Isotype
- IgG
- Antibody clone number
- 4H84
- Storage
- Store at 4°C. Do not freeze.Aliquot to avoid repeated freezing and thawing.
Submitted references Characterization of human leukocyte antigen-G (HLA-G) expression in endometrial adenocarcinoma.
A novel sandwich ELISA for alpha1 domain based detection of soluble HLA-G heavy chains.
The 4H84 monoclonal antibody detecting beta2m free nonclassical HLA-G molecules also binds to free heavy chains of classical HLA class I antigens present on activated lymphocytes.
Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients.
Barrier BF, Kendall BS, Sharpe-Timms KL, Kost ER
Gynecologic oncology 2006 Oct;103(1):25-30
Gynecologic oncology 2006 Oct;103(1):25-30
A novel sandwich ELISA for alpha1 domain based detection of soluble HLA-G heavy chains.
Juch H, Blaschitz A, Daxböck C, Rueckert C, Kofler K, Dohr G
Journal of immunological methods 2005 Dec 20;307(1-2):96-106
Journal of immunological methods 2005 Dec 20;307(1-2):96-106
The 4H84 monoclonal antibody detecting beta2m free nonclassical HLA-G molecules also binds to free heavy chains of classical HLA class I antigens present on activated lymphocytes.
Poláková K, Kuba D, Russ G
Human immunology 2004 Feb;65(2):157-62
Human immunology 2004 Feb;65(2):157-62
Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules.
Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N
Human immunology 2003 Mar;64(3):315-26
Human immunology 2003 Mar;64(3):315-26
Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients.
Poláková K, Krcová M, Kuba D, Russ G
Leukemia research 2003 Jul;27(7):643-8
Leukemia research 2003 Jul;27(7):643-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by HLA-G monoclonal antibody, clone 4H84 (Cat # MAB3858) and protein G.HLA-G was detected by HLA-G monoclonal antibody, clone 4H84 (Cat # MAB3858) and goat anti-mouse-HRP conjugate in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).
- Validation comment
- Immunoprecipitation
