335904

antibody from BioLegend

Targeting: HLA-G

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Functional assay (Recommended by provider)

Antibody Data

Product number

335904

Provider

BioLegend

Proper citation

BioLegend Cat#335904, RRID:AB_10641840

Product name

LEAF™ Purified anti-human HLA-G [87G]

Antibody type

Monoclonal

Antigen

HLA-G transfected cells

Description

HLA-G is a nonclassical MHC class I (MHC-Ib) molecule structurally related to MHC class Ia (HLA-A,B, C). There are seven isoforms of this molecule, including membrane bound HLA-G1, -G2, -G3 and -G4 and soluble HLA-G5, -G6, and -G7. HLA-G is primarily expressed on trophoblast cells. Its expression is also found on thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, and in various tumors. HLA-G exerts its inhibitory functions to regulate immune tolerance via interaction with inhibitory receptors ILT2(CD85j), ILT4(CD85d), and KIR2DL4(CD158d), which is expressed on NK cells, T cells, monocytes, dendritic cells, and B cells. HLA-G is also able to bind CD8α/α, which may mediate positive and/or negative selection in thymus.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

500 µg

Concentration

1 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human cytotrophoblastic cell line, JEG-3 was stained with LEAF™ purified HLA-G (clone 87G, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG FITC.

Conjugate

Unconjugated