Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Flow cytometry [1]
- Other assay [3]
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Validation data
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- Product number
- MA1-19014 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HLA-G Monoclonal Antibody (MEM-G/9)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody reacts with an extracellular epitope on native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. It will not cross-react with mouse.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MEM-G/9
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4°C, do not freeze
Submitted references LILRB1 Blockade Enhances Bispecific T Cell Engager Antibody-Induced Tumor Cell Killing by Effector CD8(+) T Cells.
Chymase-producing cells of the innate immune system are required for decidual vascular remodeling and fetal growth.
Kim A, Han CJ, Driver I, Olow A, Sewell AK, Zhang Z, Ouyang W, Egen JG, Yu X
Journal of immunology (Baltimore, Md. : 1950) 2019 Aug 15;203(4):1076-1087
Journal of immunology (Baltimore, Md. : 1950) 2019 Aug 15;203(4):1076-1087
Chymase-producing cells of the innate immune system are required for decidual vascular remodeling and fetal growth.
Meyer N, Woidacki K, Knöfler M, Meinhardt G, Nowak D, Velicky P, Pollheimer J, Zenclussen AC
Scientific reports 2017 Mar 22;7:45106
Scientific reports 2017 Mar 22;7:45106
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of HLA-G from HLA-G1 transfectants using HLA-G Monoclonal Antibody (MEM-G/9) (Product # MA1-19014) and protein G. HLA-G was detected by HLA-G Monoclonal Antibody and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of HLA-G using HLA-G Monoclonal Antibody (MEM-G/9) (Product # MA1-19014). Separation of HLA-G trasnfected LCL cells (red-filled) from K562 cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human HLA-G (MEM-G/9) purified antibody (concentration in sample 0.3 μg/mL, GAM APC).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HLA-G using a monoclonal antibody (Product # MA1-19014).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) and protein G. HLA-G was detected by anti-human HLA-G (clone 4H84) Monoclonal antibody (Product # MA1-19014) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) and protein G. HLA-G was detected by anti-human HLA-G (clone 4H84) Monoclonal antibody (Product # MA1-19014) and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).