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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- NB500-533 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-533, RRID:AB_10002261
- Product name
- Mouse Monoclonal HLA G Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. The antibody MEM-G/4 reacts with denaturated HLA-G heavy chain. The recognized epitope is present only on HLA-G1, -G2 and -G5 molecules. HLA-G belongs to the MHC Class I molecules (MHC Class Ib
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Notch-dependent RBPJκ inhibits proliferation of human cytotrophoblasts and their differentiation into extravillous trophoblasts.
Velicky P, Haider S, Otti GR, Fiala C, Pollheimer J, Knöfler M
Molecular human reproduction 2014 Aug;20(8):756-66
Molecular human reproduction 2014 Aug;20(8):756-66
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HLA G Antibody (MEM-G/4) [NB500-533] - Analysis of HLA-G by the antibody MEM-G/4 on HLA-G1 transfectants (LCL-HLA-G1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: HLA G Antibody (MEM-G/4) [NB500-533] - Analysis using Allophycocyanin conjugate of NB500-533. Immunofluorescence staining of HLA-G1 transfectants using anti-human HLA-G (MEM-G/9) Alexa Fluor (R) 488 Fab-fragment.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HLA G Antibody (MEM-G/4) [NB500-533] - Analysis using Allophycocyanin conjugate of NB500-533. Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC (NBP1-44915).
