MA1-19219

antibody from Invitrogen Antibodies

Targeting: HLA-G

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-19219

Provider

Invitrogen Antibodies

Product name

HLA-G Monoclonal Antibody (MEM-G/1)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This antibody reacts with an extracellular epitope of denaturated HLA-G heavy chain. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. Immunohistochemistry (Paraffin): Incubation: 1 h at RT.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

MEM-G/1

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C, do not freeze

References

Submitted references

Forkhead box C2 promotes the invasion ability of human trophoblast cells through Hedgehog (Hh) signaling pathway.

Zhang Y, Zhang Y
Cell biology international 2018 Jul;42(7):859-866

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Differential expression of β-catenin and Dickkopf-1 in the third trimester placentas from normal and preeclamptic pregnancies: a comparative study.

Zhang Z, Li H, Zhang L, Jia L, Wang P
Reproductive biology and endocrinology : RB&E 2013 Mar 4;11:17

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Association of Wnt2 and sFRP4 expression in the third trimester placenta in women with severe preeclampsia.

Zhang Z, Zhang L, Zhang L, Jia L, Wang P, Gao Y
Reproductive sciences (Thousand Oaks, Calif.) 2013 Aug;20(8):981-9

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blotting analysis of human HLA-G using mouse monoclonal antibody MEM-G/1 on lysates of JEG-3 cell line and LNCaP cell line (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse Monoclonal antibody anti-HLA-G (Product # MA1-19219) followed by IRDye800-conjugated anti-mouse secondary antibody. HLA-G was detected at approximately 40kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blotting analysis of human HLA-G using mouse monoclonal antibody MEM-G/1 on lysates of JEG-3 cell line and LNCaP cell line (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse Monoclonal antibody anti-HLA-G (Product # MA1-19219) followed by IRDye800-conjugated anti-mouse secondary antibody. HLA-G was detected at approximately 40kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Immunostaining of beta-catenin in the placental tissue sections from normal and severe preeclampsia groups. Figure 3 A1 and A3 show the immunostaining of the beta-catenin in normal control (N) group; A2 and A4 show the immunostaining of the beta-catenin in severe preeclampsia (sPE) group. The staining intensity of beta-catenin in the placental tissue of the severe PE group (A2 and A4) was weaker than the control group (A1 and A3). B1 and B2: serial sections of A3 and A4 stained with HLA-G. STB = syncytiotrophoblast, EVT = extravillous trophoblast. Original magnification: 400x for A1 and A2, 200x for A3, A4, B1, B2.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Immunostaining of DKK1 in the placental tissue sections from normal and severe preeclampsia groups. Figure 4 A1 and A3 show the immunostaining of the DKK1 in normal control (N) group; A2 and A4 show the immunostaining of the DKK1 in severe preeclampsia (sPE) group. The staining intensity of DKK1 in the placental tissue of the severe PE group (A2 and A4) was greater than the control group (A1 and A3). B1 and B2: serial sections of A3 and A4 stained with HLA-G. STB = syncytiotrophoblast, EVT = extravillous trophoblast. Original magnification: 400x for A1 and A2, 200x for A3, A4, B1, B2.