Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Flow cytometry [4]
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Validation data
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- Product number
- NB500-314 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-314, RRID:AB_10002354
- Product name
- Mouse Monoclonal HLA G Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. The antibody MEM-G/9 reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
- Reactivity
- Human, Mouse
- Host
- Mouse
- Antigen sequence
aa 16-25 of human p53
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references HLA-G regulates the invasive properties of JEG-3 choriocarcinoma cells by controlling STAT3 activation.
Insulin-secreting cells from human eyelid-derived stem cells alleviate type I diabetes in immunocompetent mice.
Liu X, Gu W, Li X
Placenta 2013 Nov;34(11):1044-52
Placenta 2013 Nov;34(11):1044-52
Insulin-secreting cells from human eyelid-derived stem cells alleviate type I diabetes in immunocompetent mice.
Kang HM, Kim J, Park S, Kim J, Kim H, Kim KS, Lee EJ, Seo SI, Kang SG, Lee JE, Lim H
Stem cells (Dayton, Ohio) 2009 Aug;27(8):1999-2008
Stem cells (Dayton, Ohio) 2009 Aug;27(8):1999-2008
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: HLA G Antibody (MEM-G/9) [NB500-314] - Analysis using the HRP conjugate of NB500-314. Detection of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) and protein G. HLA-G was detected by anti-human HLA-G (4H84)and goat anti-mouse HRP in cell lysate (Lanes 1) and in the immunoprecipitate (Lanes 2).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Analysis using the HRP conjugate of NB500-314. Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Analysis of FITC conjugate of NB500-314. Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) FITC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HLA G Antibody (MEM-G/9) [NB500-314] - Surface staining of HLA-G1 transfectants (LCL-HLA-G1) using anti-HLA-G (MEM-G/9) PE.