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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA503550 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503550, RRID:AB_11126098
- Product name
- RAD51L1 mouse monoclonal antibody, clone OTI7H4 (formerly 7H4)
- Antibody type
- Monoclonal
- Description
- RAD51L1 mouse monoclonal antibody, clone OTI7H4 (formerly 7H4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- RAD51B
- Isotype
- IgG
- Antibody clone number
- OTI7H4
- Vial size
- 100 µl
- Concentration
- 0.27 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD51L1 (RC206457, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD51L1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-RAD51L1 monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-RAD51L1 mouse monoclonal antibody (TA503550) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RAD51L1(RC206457).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC206457 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RAD51L1 antibody(TA503550), and then analyzed by flow cytometry.
- Validation comment
- FC
