PA5-112107

antibody from Invitrogen Antibodies

Targeting: CTAGE1 CT21.1, CT21.2, CTAGE, cTAGE-1, cTAGE-2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112107

Provider

Invitrogen Antibodies

Product name

CTAGE1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CTAGE1 using CTAGE1 Polyclonal Antibody (Product # PA5-112107) at a dilution of 1:500. Lane A: MDA-MB453 whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG H&L (Dylight 800) at 1:10,000 dilution. Developed using the Odyssey technique and performed under reducing conditions. Predicted band size: 85 kDa. Observed band size: 85 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of CTAGE1 was performed on (Lane A) 0.5 mg 293T whole cell lysate and (Lane B) 0.5 mg Hela whole cell lysate, using 0.5 µL of CTAGE1 Polyclonal Antibody (Product # PA5-112107) at a dilution of 1:500 and 15 µL of 50 % Protein G agarose. A Dylight 800-labeled antibody to rabbit IgG (H+L) was used as a secondary antibody at a 1:5000 dilution. Developed using the odssey technique and performed under reducing conditions. Predicted band size: 85 kDa. Observed band size: 85 kDa.