PA5-51501

antibody from Invitrogen Antibodies

Targeting: PHF6 BFLS, BORJ, CENP-31, KIAA1823, MGC14797

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-51501

Provider

Invitrogen Antibodies

Product name

PHF6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MSSSVEQKKG PTRQRKCGFC KSNRDKECGQ LLISENQKVA AHHKCMLFSS ALVSSHSDNE SLGGFSIEDV QKEIKRGTKL MCSLCHCPGA TIGCDVKTCH RTYHYHCALH DK Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 98%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PHF6 in Lane 1: Marker (kDa) 219, 112, 85, 49, 32, 25, 18; Lane 2: Human cell line RT-4; Lane 3: Human cell line EFO-21; Lane 4: Human cell line A-431. Samples were probed using a PHF6 Polyclonal Antibody (Product # PA5-51501).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PHF6 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a PHF6 Polyclonal Antibody (Product # PA5-51501).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of PHF6 in human cell line U-251 MG shows positivity in nucleus & nucleoli. Samples were probed using a PHF6 Polyclonal Antibody (Product # PA5-51501).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PHF6 in human ovary using a PHF6 Polyclonal Antibody (Product # PA5-51501) shows strong nuclear positivity in follicle and ovarian stroma cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PHF6 in human epididymis using PHF6 Polyclonal Antibody (Product # PA5-51501) shows high expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PHF6 in human liver using PHF6 Polyclonal Antibody (Product # PA5-51501) shows low expression as expected.