Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-68260 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-68260, RRID:AB_1109815
- Product name
- Rabbit Polyclonal PHF6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PHF6 Antibody [NB100-68260] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PHF6 antibody NB100-68260 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68261 and NB100-68262, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68260] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PHF6 used at a dilution of 1:200 (1ug/ml). Detection: DAB