Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00000466-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000466-M03, RRID:AB_1144833
- Product name
- Mouse Monoclonal ATF1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ATF1 - activating transcription factor 1 (3E7)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATF1 Antibody (3E7) [H00000466-M03] - Western blot analysis of ATF1 over-expressed 293 cell line. Lane 2 cotransfected with ATF1 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000466-M03. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATF1 Antibody (3E7) [H00000466-M03] - Analysis of ATF1 expression in transfected 293T cell line by ATF1 monoclonal antibody (M03), clone 3E7. Lane 1: ATF1 transfected lysatE (29.2 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ATF1 Antibody (3E7) [H00000466-M03] - Detection limit for recombinant GST tagged ATF1 is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ATF1 Antibody (3E7) [H00000466-M03] - Analysis of monoclonal antibody to ATF1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml
