Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- NBP1-52647 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-52647, RRID:AB_11018361
- Product name
- Rabbit Polyclonal ATF1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATF1 Antibody [NBP1-52647] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-ATF1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ATF1 was also immunoprecipitated by rabbit anti-ATF1 antibodies NBP1-52645 and NBP1-52646, which recognize upstream epitopes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATF1 Antibody [NBP1-52647] - Detection of Human ATF1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATF1 antibody NBP1-52647 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.