LS-C343871

antibody from LSBio

Targeting: MT-CO1 COI, COX1, MTCO1

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C343871

Provider

LSBio

Product name

MT-CO1 / COX1 Antibody (aa501-514) LS-C343871

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MTCO1 antibody Western blot. Lane 1: SMMC Cell Lysate. Lane 2: MCF-7 Cell Lysate. Lane 3: RAJI Cell Lysate. Lane 4: SW620 Cell Lysate.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of MTCO1 using anti-MTCO1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The samples were loaded under reducing conditions. Lane 1: human HeLa mitochondria lysates at 20ug, Lane 2: human HeLa whole cell lysates at 20ug, Lane 3: human Caco-2 whole cell lysates at 50ug, Lane 4: rat heart tissue lysates at 50ug. Lane 5: mouse heart tissue lysates at 50ug. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 60 minutes. Blocked the membrane with 5% Non-fat Milk in TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTCO1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTCO1 at approximately 37KD. The expected band size for MTCO1 is at 57KD.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MTCO1 antibody ICC: C6 Cell.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

MTCO1 antibody ICC: C6 Cell.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

MTCO1 antibody ICC: C6 Cell.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MTCO1 antibody IHC-paraffin: Human Mammary Cancer Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

MTCO1 antibody IHC-paraffin: Human Mammary Cancer Tissue.