Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-64411 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MTCO1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LIRAELGQPG NLLGNDHIYN VIV Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 87%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MTCO1 Polyclonal Antibody(Product # PA5-64411) and a 52kDa band corresponding to MTCO1 was observed across the panel tested. Membrane enriched extracts (30 µg lysate) of A549 (Lane 1), A549 treated with Cisplatin (12uM for 24 hr) (Lane 2), Hep G2 (Lane 3), SH-SY5Y (Lane 4), HeLa (Lane 5), OVCAR-3 (Lane 6), PANC-1 (Lane 7) and BeWo (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of COX1 in human heart muscle using COX1 Polyclonal Antibody (Product # PA5-64411) shows positivity in cardiomyocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of COX1 in human kidney using COX1 Polyclonal Antibody (Product # PA5-64411) shows granular cytoplasmic positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of COX1 in human cerebral cortex using COX1 Polyclonal Antibody (Product # PA5-64411) shows positivity in neurons.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of COX1 in human liver using COX1 Polyclonal Antibody (Product # PA5-64411) shows positivity in hepatocytes.