F49620

antibody from NSJ Bioreagents

Targeting: PRKN AR-JP, PARK2, parkin, PDJ

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F49620

Provider

NSJ Bioreagents

Product name

Parkin Antibody / PARK2

Antibody type

Polyclonal

Description

This highly specific Parkin antibody is suitable for use in Immunofluorescence/Immunohistochemistry/Western blot/Flow cytometry/ELISA applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Parkin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of lysate from (1) SH-SY5Y cell line and (2) human brain tissue lysate using Parkin antibody at 1:1000. Predicted molecular weight: 50-60 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of Parkin antibody with NCI-H460 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Parkin antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Parkin antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.