PA5-13399

antibody from Invitrogen Antibodies

Targeting: PRKN AR-JP, PARK2, parkin, PDJ

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-13399

Provider

Invitrogen Antibodies

Product name

Parkin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Mutated FANCA Gene Role in the Modulation of Energy Metabolism and Mitochondrial Dynamics in Head and Neck Squamous Cell Carcinoma.

Bertola N, Degan P, Cappelli E, Ravera S
Cells 2022 Jul 30;11(15)

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Mitochondrial fission and mitophagy are independent mechanisms regulating ischemia/reperfusion injury in primary neurons.

Anzell AR, Fogo GM, Gurm Z, Raghunayakula S, Wider JM, Maheras KJ, Emaus KJ, Bryson TD, Wang M, Neumar RW, Przyklenk K, Sanderson TH
Cell death & disease 2021 May 12;12(5):475

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NCI-H460 cells using a PARK2 polyclonal antibody (Product # PA5-13399) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Actin filaments were stained with dye-conjugated phalloidin (red). Nuclei were stained with DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of NCI-H460 cells using a PARK2 polyclonal antibody (Product # PA5-13399) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 PINK1/Parkin-mediated mitophagy. A Cells labeled with PINK1 and ATPB for colocalization of PINK1 with mitochondria, n = 4. B Cells labeled with Parkin and ATPB for colocalization of Parkin with mitochondria, n = 3. C Quantification of colocalization using Pearson''s correlation coefficient (PCC). D Western Blot of mitochondrial and cytosolic fractions for PINK1, Parkin, and ubiquitin. E Quantitation of protein levels, normalized to VDAC and GAPDH, Ubiquitin: n = 6, PINK1: n = 5, Parkin: n = 8. F Western Blot of LC3 (autophagy marker) and Rab5 (endosome marker) in mitochondrial and cytosolic fractions. G Quantitation of LC3 conversion and Rab5, normalized to VDAC and GAPDH, LC3: n = 4, Rab5: n = 4. Differences between groups were computed using one-way ANOVA with Dunnett''s post-hoc analysis for comparisons versus control. R post-reoxygenation; * p < 0.05; ** p < 0.01; *** p < 0.001. Scale bar = 10 um.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression evaluation of proteins involved in mitophagy and autophagy processes in OHSU-974-FAcorr and OHSU-974-S91. ( A ) Western blot signals of Pink1 and Parkin (two mitophagy markers), Beclin1, Atg7, Atg12, Atg16L1, LC3 (autophagy markers), and beta-Actin. ( B ) Densitometric analysis of WB signals reported in Panel A, normalized versus beta-Actin. Data in histograms are reported as mean +- SD and are representative of at least 3 independent experiments. Statistical significance was tested with an unpaired t -test. * represents a p < 0.05 between OHSU-974-S91 cells and the OHSU-974-FAcorr cells used as control.