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- References [1]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- GTX29769 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX29769, RRID:AB_370828
- Product name
- GSK3 beta (phospho Ser9) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Drosophila
- Host
- Rabbit
Submitted references Interaction between eye pigment genes and tau-induced neurodegeneration in Drosophila melanogaster.
Ambegaokar SS, Jackson GR
Genetics 2010 Sep;186(1):435-42
Genetics 2010 Sep;186(1):435-42
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rabbit polyclonal to phospho GSK beta (Ser 9) at 1/1000 on 293T cell extract shows a band at49 kD. Cells were serum starved for 24 hours: Lane 1 : control; Lane 2 : treated with IGF-1 (100 ng/ml) for 20 min; Lane 3 : pre-treated with 10 uM LY294002 (s
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex Immunochemical's Rabbit-anti-GSK3B pS9 antibody at a 1:1,000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 kDa. Cells were serum starved for 24 h prior to extraction. Lane 1: Control, Lane 2: treated with IGF-1 (100 ng/ml) for 20' , lane 3: pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20'. Molecular weight markers confirm a MW of ~ 49 kDa.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rabbit anti-GSK3B pS9 (GTX29769) was used at a 1:200 dilution to detect GSK3B by immunohistochemistry in human ovarian cancer tumor tissue. Tissue was formalin-fixed and paraffin embedded.
