Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AST-022-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SVCT2/SLC23A2 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4)brain lysates: - 1,2. Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022), (1:200).3,4. Anti-SVCT2/SLC23A2 (extracellular) Antibody , preincubated with SVCT2/SLC23A2 (extracellular) Blocking Peptide (#BLP-ST022).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4)brain lysates: - 1,2. Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022), (1:200).3,4. Anti-SVCT2/SLC23A2 (extracellular) Antibody , preincubated with SVCT2/SLC23A2 (extracellular) Blocking Peptide (#BLP-ST022).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human MCF-7 breast adenocarcinoma (lanes 1 and 3) and human COLO 205 colon adenocarcinoma (lanes 2 and 4)cell line lysates: - 1,2. Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022), (1:200).3,4. Anti-SVCT2/SLC23A2 (extracellular) Antibody , preincubated with SVCT2/SLC23A2 (extracellular) Blocking Peptide (#BLP-ST022).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of SVCT2 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. SVCT2 staining (green) in CA1 hippocampal region is detected in axonal processes (arrows), with the highest density surrounding the pyramidal layer (P). Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of SVCT2 in live intact human Jurkat T-cell leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-SVCT2/SLC23A2 (extracellular) Antibody (#AST-022), (5 µg) + goat- Anti-rabbit-FITC.