PA1-29466

antibody from Invitrogen Antibodies

Targeting: CXCR1 CD181, CDw128a, CKR-1, CMKAR1, IL8RA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-29466

Provider

Invitrogen Antibodies

Product name

CXCR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is also expected to react with chimpanzee, gorilla, and rabbit due to sequence homology. Predicted molecular weight: 42 kDa.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CXCR1 was performed by loading 15 µg of RAW264.7 cell lysate. Proteins were transferred to a membrane and probed with a CXCR1 Polyclonal Antibody (Product # PA1-29466) at a dilution of 2.0 µg/mL.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of C-X-C chemokine receptor type 1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CXCR1 Polyclonal Antibody (Product # PA1-29466) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing plasma membrane and cytoplasm localization. Panel e represents negative/low expressing cells Hep G2. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.