14-1819-82

antibody from Invitrogen Antibodies
Targeting: CXCR1 CD181, CDw128a, CKR-1, CMKAR1, IL8RA
Provider product page for 14-1819-82

Antibody data

Product number
14-1819-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD181 (CXCR1) Monoclonal Antibody (eBio8F1-1-4 (8F1-1-4)), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The eBio8F1-1-4 monoclonal antibody reacts with human CD181 (CXCR1, IL-8Ralpha). CD181 is a 67-70 kDa member of the 7-transmembrane spanning G-protein coupled receptor (GPCR) family. CD181 is expressed as a homodimer, or a heterodimer with CD182 (CXCR2, IL-8Rbeta) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of it's ligands, which include IL-8, NAP-2, GCP-2 and GRO-alpha, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181. Applications Reported: This eBio8F1-1-4 (8F1-1-4) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio8F1-1-4 (8F1-1-4) antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
eBio8F1-1-4 (8F1-1-4)
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
CXCR1 protein structure - 14-1819-82 shown in red.