14-1819-82
antibody from Invitrogen Antibodies
Targeting: CXCR1
CD181, CDw128a, CKR-1, CMKAR1, IL8RA
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- 14-1819-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD181 (CXCR1) Monoclonal Antibody (eBio8F1-1-4 (8F1-1-4)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBio8F1-1-4 monoclonal antibody reacts with human CD181 (CXCR1, IL-8Ralpha). CD181 is a 67-70 kDa member of the 7-transmembrane spanning G-protein coupled receptor (GPCR) family. CD181 is expressed as a homodimer, or a heterodimer with CD182 (CXCR2, IL-8Rbeta) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of it's ligands, which include IL-8, NAP-2, GCP-2 and GRO-alpha, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181. Applications Reported: This eBio8F1-1-4 (8F1-1-4) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio8F1-1-4 (8F1-1-4) antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBio8F1-1-4 (8F1-1-4)
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.
Granulocyte chemotactic protein 2 acts via both IL-8 receptors, CXCR1 and CXCR2.
Wu B, Wang L, Jiang L, Dong L, Xu F, Lu Y, Jin J, Wang Z, Liang G, Shan X
Molecular medicine reports 2018 Jan;17(1):179-185
Molecular medicine reports 2018 Jan;17(1):179-185
Granulocyte chemotactic protein 2 acts via both IL-8 receptors, CXCR1 and CXCR2.
Wolf M, Delgado MB, Jones SA, Dewald B, Clark-Lewis I, Baggiolini M
European journal of immunology 1998 Jan;28(1):164-70
European journal of immunology 1998 Jan;28(1):164-70
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG2b kappa Isotype Control Purified (Product # 14-4732-82) (left) or 0.25 µg of Anti-Human CD181 (CXCR1) Purified (right) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Cells in the large scatter population were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL