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- Product number
- MA1-206 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCR1 Monoclonal Antibody (501)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA1-206 detects CXCR1/IL8RA from human samples.
- Antibody clone number
- 501
- Concentration
- 1 mg/mL
Submitted references Overexpression of BQ323636.1 Modulated AR/IL-8/CXCR1 Axis to Confer Tamoxifen Resistance in ER-Positive Breast Cancer.
Tsoi H, Shi L, Leung MH, Man EPS, So ZQ, Chan WL, Khoo US
Life (Basel, Switzerland) 2022 Jan 10;12(1)
Life (Basel, Switzerland) 2022 Jan 10;12(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CXCR1/IL8RA was performed by loading 20 µg of indicated whole cell lysates in reducing sample buffer and 8 µL PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX). Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk/TBST for at least 1 hour at room temperature. CXCR1/IL8RA was detected using a CXCR1/IL8RA mouse monoclonal antibody (Product # MA1-206) at a concentration of 1 µg/mL in blocking buffer overnight at 4°C on a rocking platform, followed by a HRP conjugated secondary antibody (Product # A16078) at a dilution of 1:5000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Dura Extended Duration Substrate (Product # 34076) and the myECL Imager (Product # 62236).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CXCR1/IL8RA (green) in MCF7 cells. Cells were blocked with blocking buffer (Product # 37525) for 30 min at room temparture and then stained with CXCR1/IL8RA mouse monoclonal antibody (Product # MA1-206) at a dilution of 1:100 for 1 hour at room temperature. After washing with PBS for 3 times, the cells were then stained with Alexa Fluor 488 goat anti-mouse secondary antibody (Product # A28175) for 1 hour at room temperature. The cells were fixed with 4% paraformaldehyde for 30 minutes at room temperature. Nuclei (blue) were counterstained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific EVOS FL Cell Imaging System at 40X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CXCR1/IL8RA on Raji cells. Cells were blocked with blocking buffer (Product # 37525) for 30 min at room temparture and then stained with CXCR1/IL8RA mouse monoclonal antibody (Product # MA1-206) at a dilution as indicated for 1 hour on ice. After washing with ice-cold PBS for 3 times, the cells were stained with Alexa Fluor 488 goat anti-mouse secondary antibody (Product # A28175) for 1 hour on ice. A representative 10,000 cells were acquired for each sample.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8 Clinical significance of CXCR1 in breast cancer. ( a ) Immunohistochemistry of BQ and CXCR2 was performed on primary ER+ve breast tumor on TMA. ( b ) Chi-square test to determine the correlation between nuclear BQ and cytoplasmic CXCR1. ( c ) Tamoxifen resistance was associated with high expression of cytoplasmic CXCR1. Chi-square test and Mann-Whitney U test were employed. * represents p < 0.05. Chi-square test to determine the correlation of cytoplasmic CXCR1 with ( d ) relapse and ( e ) metastasis. High expression of CXCR1 was associated with poorer ( f ) overall survival and ( g ) disease-specific survival in ER+ve breast cancer. Log-rank test was employed.
