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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27184 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HepG2, mouse leukocyte treated with CFA. Predicted reactivity: Chimpanzee (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.97 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CXCR1/IL8RA using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CXCR1/IL8RA polyclonal antibody (Product # PA5-27184) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CXCR1 was performed by separating 50 µg of untreated (–) and treated (+) mouse tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CXCR1 Polyclonal Antibody (Product # PA5-27184) at a dilution of 1:500. (CFA: Complete Freunds adjuvant).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CXCR1/IL8RA in methanol-fixed Hep G2 cells using a CXCR1/IL8RA polyclonal antibody (Product # PA5-27184) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCR1 Polyclonal Antibody detects CXCR1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CXCR1 stained by CXCR1 Polyclonal Antibody (Product # PA5-27184) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCR1 Polyclonal Antibody detects IL8RA protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. CXCR1 Polyclonal Antibody (Product # PA5-27184) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
