PA5-22314

antibody from Invitrogen Antibodies

Targeting: ALYREF ALY, ALY/REF, BEF, REF, THOC4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22314

Provider

Invitrogen Antibodies

Product name

ALY Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain. Predicted reactivity: Mouse (96%), Rat (98%), Chicken (82%), Rhesus Monkey (98%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.63 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ALY Polyclonal Antibody (Product # PA5-22314). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. ALY Polyclonal Antibody (Product # PA5-22314) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ALY was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a ALY Polyclonal Antibody (Product # PA5-22314) at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ALY in paraformaldehyde-fixed HeLa cells using an ALY polyclonal antibody (Product # PA5-22314) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of ALY was performed in paraffin-embedded human cervical carcinoma tissue using ALY Polyclonal Antibody (Product # PA5-22314) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of ALY was performed in paraffin-embedded human esophageal cancer tissue using ALY Polyclonal Antibody (Product # PA5-22314) at a dilution of 1:500.