Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PB9321 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-PKC iota/PRKCI Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for PKC IOTA/PRKCI detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. PKC IOTA/PRKCI information: Molecular Weight: 68262 MW; Subcellular Localization: Cytoplasm. Membrane. Endosome. Nucleus. Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by SRC, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Transported to vesicular tubular clusters (VTCs) through interaction with RAB2A; Tissue Specificity: Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Anti- PKC iota Picoband antibody, PB9321, Western blottingAll lanes: Anti PKC iota (PB9321) at 0.5ug/mlLane 1: SHG Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: U87 Whole Cell Lysate at 40ugLane 4: 293T Whole Cell Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugLane 6: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 68KDObserved bind size: 68KD
- Additional image