A303-895A

antibody from Invitrogen Antibodies

Targeting: HNRNPL HNRPL

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A303-895A

Provider

Invitrogen Antibodies

Product name

hnRNP-L Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human hnRNP-L by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-hnRNP-L antibody A303-895A (lot A303-895A-1) used for IP at 6 µg/mg lysate. hnRNP-L was also immunoprecipitated by rabbit anti-hnRNP-L antibody A303-896A. For blotting immunoprecipitated hnRNP-L, A303-895A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse hnRNP-L by western blot. Samples: Whole cell lysate from mouse NIH3T3 (15 and 50 µg) cells. Antibodies: Affinity purified rabbit anti-hnRNP-L antibody A303-895A (lot A303-895A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human hnRNP-L by western blot. Samples: Whole cell lysate from 293T (15 and 50 µg), HeLa (50µg), and Jurkat (50µg) cells. Antibodies: Affinity purified rabbit anti-hnRNP-L antibody A303-895A (lot A303-895A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse hnRNP-L by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- hnRNP-L (Cat. No. A303-895A Lot1) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human hnRNP-L by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- hnRNP-L (Cat. No. A303-895A Lot1) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.