10328-1-AP

antibody from Invitrogen Antibodies

Targeting: NXF1 DKFZp667O0311, Mex67, TAP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10328-1-AP

Provider

Invitrogen Antibodies

Product name

NXF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SGIRSSRLE EDDGDVAMSD AQDGPRVRYN PYTTRPNRRG DTWHDRDRIH VTVRRDRAPP ERGGAGTSQD GTSKNWFKIT IPYGRKYDKA WLLSMIQSKC SVPFTPIEFH YENTRAQFFV EDASTASALK AVNYKILDRE NRRISIIINS SAPPHTILNE LKPEQVEQLK LIMSKRYDGS QQALDLKGLR SDPDLVAQNI DVVLNRRSCM AATLRIIEEN IPELLSLNLS NNRLYRLDDM SSIVQKAPNL KILNLSGNEL KSERELDKIK GLKLEELWLD GNSLCDTFRD QSTYISAIRE RFPKLL (45-349 aa encoded by BC004904)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of NXF1 antibody (10328-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NXF1 transfected HeLa cells.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 10328-1-AP (NXF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-NXF1 (IP:10328-1-AP, 4ug; Detection:10328-1-AP 1:500) with HeLa cells lysate 3000 ug.