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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-21412 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLaS3, HepG2, Molt-4, Raji, mouse brain. Predicted reactivity: Mouse (98%), Rat (97%), Pig (96%), Chimpanzee (100%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 10 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TAP was performed by separating 30 µg of A431 lysates by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a TAP Polyclonal Antibody (Product # PA5-21412) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TAP Polyclonal Antibody (Product # PA5-21412). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. TAP Polyclonal Antibody (Product # PA5-21412) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TAP in paraformaldehyde-fixed HeLa cells using a TAP polyclonal antibody (Product # PA5-21412) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TAP (Product # PA5-21412) antibody at 1:200 dilution.
