PA5-96229

antibody from Invitrogen Antibodies

Targeting: ADCYAP1R1 PAC1, PAC1R, PACAPR

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96229

Provider

Invitrogen Antibodies

Product name

PACAP Receptor Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: HeLa, SH-SY5Y, U-87MG; Cellular Location: Cell membrane, Multi-pass membrane protein Immunogen sequence: CIFKKEQAMC LEKIQRANEL MGFNDSSPGC PGMWDNITCW KPAHVGEMVL VSCPELFRIF NPDQVWETET IGESDFGDSN SLDLSDMGVV SRNCTEDGWS EPFPHYFDAC GFDEYE

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.84 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PACAP Receptor in extracts of Mouse heart cells. Samples were incubated with PACAP Receptor Polyclonal antibody (Product # PA5-96229) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PACAP Receptor in extracts of U-87MG cells. Samples were incubated with PACAP Receptor Polyclonal antibody (Product # PA5-96229) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PACAP Receptor in NIH/3T3 cells. Samples were incubated with PACAP Receptor Polyclonal antibody (Product # PA5-96229) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PACAP Receptor in SH-SY5Y cells. Samples were incubated with PACAP Receptor Polyclonal antibody (Product # PA5-96229) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PACAP Receptor in C6 cells. Samples were incubated with PACAP Receptor Polyclonal antibody (Product # PA5-96229) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.