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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AP02276PU-N - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-Collagen type VII antibody, Ig Fraction
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-Collagen type VII antibody, Ig Fraction
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- Col7a1
- Vial size
- 0.1mg
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 1. Western Blot analysis using AP02276PU Collagen VII antibody. Extract of Murine dermis, recombinant GST-mCol7 (aa 757-967) and GST were separated by SDS-PAGE and Immunoblotted with Immune and Preimmune Serum preadsorbed against GST. IgG from Immune Serum binds cell-derived (arrow) and recombinant fragment (arrow head) of Collagen type VII. Sitaru C et al. (2005). J. Clin. Invest. 115 (4):870-8.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 2. Immunofluorescence of Collagen VII staining in Cryosections of Murine (A) and Human Skin (B). This antibody AP02276PU stains the Dermal-Epidermal Junction (DEJ). Original magnification 250x. Sitaru C et al. (2005). J. Clin. Invest. 115 (4):870-8.
- Validation comment
- IF
