PA1-12529

antibody from Invitrogen Antibodies

Targeting: BACE1 BACE

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-12529

Provider

Invitrogen Antibodies

Product name

Anti-BACE1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

In Western blot, this antibody detects a ~70 kDa band in human brain lysate.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using BACE1 Polyclonal Antibody (Product # PA1-12529) and a 55 kDa band corresponding to BACE1 was observed across cell lines and tissues tested except Daudi, MOLT-4, Mouse Liver and Mouse Heart. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), U-87 MG (Lane 2), SH-SY5Y (Lane 3), Daudi (Lane 4), MOLT-4 (Lane 5), Mouse Brain (Lane 6), Rat Brain (Lane 7), Mouse Pancreas (Lane 8), Rat Pancreas (Lane 9), Mouse Liver (Lane 10) and Mouse Heart (lane 11) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..