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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB12256 - Provider product page
- Provider
- Abnova Corporation
- Product name
- BACE1 monoclonal antibody, clone 3C1C3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant human BACE1.
- Isotype
- IgG
- Antibody clone number
- 3C1C3
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of human BACE1 (AA: 112-324) recombinant protein (Expected MW is 49.9 kDa) with BACE1 monoclonal antibody, clone 3C1C3 (Cat# MAB12256) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [BACE1 (AA: 112-324)-hIgGFc transfected HEK293 cells] with BACE1 monoclonal antibody, clone 3C1C3 (Cat# MAB12256) at 1:500-1:2000 dilution.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Hela cell lysate; Lane 2: SK-N-SH cell lysate; Lane 3: HepG2 cell lysate; Lane 4: C6 cell lysate; Lane 5: PC-12 cell lysate; Lane 6: PANC-1 cell lysate; Lane 7: NIH/3T3 cell lysate with BACE1 monoclonal antibody, clone 3C1C3 (Cat# MAB12256) at 1:500-1:2000 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA analysis of BACE1 monoclonal antibody, clone 3C1C3 (Cat# MAB12256) at 1:10000 dilution.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MCF-7 cells with BACE1 monoclonal antibody, clone 3C1C3 (Cat# MAB12256) at 1:200-1:1000 dilution.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Hela cells with BACE1 monoclonal antibody, clone 3C1C3 (Cat# MAB12256) at 1:200-1:400 dilution (Green) and negative control (Red).
- Validation comment
- Flow Cytometry
