MA1-177

antibody from Invitrogen Antibodies

Targeting: BACE1 BACE

Western blot

Antibody data

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Validation data

Product number: MA1-177 - Provider product page
Provider: Invitrogen Antibodies
Product name: BACE1 Monoclonal Antibody (3C1C3)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA1-177 targets BACE1 in WB and IF, shows reactivity with Human, Primate, Canine, Mouse and Rat samples.
Reactivity: Human, Mouse, Rat, Canine
Host: Mouse
Isotype: IgG
Antibody clone number: 3C1C3
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

BACE1 protein structure - MA1-177 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of BACE1 (green) in HeLa cells. The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 5% Normal Goat Serum (Product # 31872) for 15 minutes at room temperature. Cells were stained with or without BACE1 monoclonal antibody (Product # MA1-177), at a dilution of 1:50 overnight at 4C, and then incubated with a DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35503) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of BACE1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with BACE1 Monoclonal Antibody (3C1C3) (Product # MA1-177) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing cytoplasmic localization. The images were captured at 60X magnification.