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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 12154-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LMBR1L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: RMFSVTGKL LVKPRLLEDL EEQLYCSAFE EAALTRRICN PTSCWLPLDM ELLHRQVLAL QTQRVLLEKR RKASAWQRNL GYPLAMLCLL VLTGLSVLIV AIHILELLID EAAMPRGMQG TSLGQVSFSK LGSFGAVIQV VLIFYLMVSS VVGFYSSPLF RSLRPRWHDT AMTQIIGNCV CLLVLSSALP VFSRTLGLTR FDLLGDFGRF NWLGNFYIVF LYNAAFAGLT TLCLVKTFTA AVRAELIRAF GLDRLPLPVS GFPQASRKTQ HQ (219-490 aa encoded by BC015015)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat testis tissue were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 A549 cells were stained with 0.2ug LMBR1L antibody (12154-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-LMBR1L (IP:12154-1-AP, 4ug; Detection:12154-1-AP 1:500) with HepG2 cells lysate 3200ug.
